This chapter presents a new approach of a quantitative analysis used to research the understanding of visual literacy issues. The objective of the research is to find common patterns, opinions, and behaviors between different people regarding the use of visual communication and people’s state of visual literacy, while also considering the possible cultural differences related. To explain visual literacy and its implications, the theoretical background about the visual literacy research field is presented first. Then, also within the section on background, the chapter presents the main concepts related to culture, and how it and visual literacy can be analyzed together to enable cross-cultural analysis. To conduct these cross-cultural analyses, this chapter proposes a new kind of quantitative questionnaire-based instrument that includes a section to measure the cultural characteristics of the individual and their level of literacy. This instrument proposal is the main result, since the research field of visual literacy lacks this kind of quantitative approach.